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You have created a recombinant DNA molecule by ligating a gene to a plasmid vector.

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You have created a recombinant DNA molecule by ligating a gene to a plasmid vector. By mistake, your friend adds exonuclease enzyme to the tube containing the recombinant DNA. How will your experiment get affected as you plan to go for transformation now?

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The experiment will not likely be affected as recombinant DNA molecule is circular and closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of DNA.

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