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a. Mark the positive and negative terminals. b. What is the charge carried by DNA molecule and how does it help in its separation?

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a. Mark the positive and negative terminals.

b. What is the charge carried by DNA molecule and how does it help in its separation?

c. How the separated DNA fragments are finally isolated?

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a.

Positive terminal – ‘B’

Negative terminal – ‘A’

b. DNA is negatively charged. Because of its negative charge, DNA moves towards the positive electrode (anode).

c. The separated DNA fragments are separated by elution. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece.

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