Correct Answer - B
Polyethylene Glycol (PEG) and sodium nitrate enhances fusion of protoplasts (protoplast fusion),Protoplast fustion is a physical phenomen which occurs due to the fusion is a physical phenomen which occurs due to the fusion of proplast (cell without cell wall) of two more cells. It can be done by mechnaically (spontaneous) or by inducing agents.
(i) Spontaneous agents Mechanical fustion
(ii) Induciing agents `NaNO_(3)` treatment.
(iii) High pH or `Ca^(2+)` treatment.
(iv) PEG treatment
(v) Electrofusion