Question

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reason could be
A. human gene may have intron which bacteria cannot process
B. amino acid codons for humans and bacteria are different
C. human protein is formed but degrated by bacteria
D. all of the above

Answer 1

Correct Answer - A
Eukaryotic genes do not function properly when transferred into bacterial cell because introns are present in eukaryotic cells but are absent in prokaryotic cells. Hence, when bacterial cell is transformed with recombinant DNA, which is generated using human gene, it could not process it. As a result, no desired protein will be produced.