Question

Which of the given statements is correct in the context of observing DNA separated by agarose gel electrophoresis ?
A. DNA can be seen in visible light.
B. DNA can be seen without staining in visible light.
C. Ethidium bromide stained DNA can be seen in visible light.
D. Ethidium bromide stained DNA can b e seen under exposure to UV light.

Answer 1

The separarted DNA fragments by the process of gel electrophoresis are visulaised after staining the DNA with ethiduium bromide followed by exposure to UV-radiation These fragments are seen as orange coloured bands.