Question

cDNA libraries have been prepared that have the same sequences as the mRNA molecules produced by organisms, or else cDNA copies are sequenced directly by RT-PCR (copying of mRNA by reverse transcriptase followed by sequencing of the cDNA copy by the polymerase chain reaction).

(a) True

(b) False

The question was posed to me by my college professor while I was bunking the class.

My question is based upon Sequencing cDNA Libraries of Expressed Genes, Submission of Sequences to the Databases in portion Collecting & Storing Sequences in Laboratory of Bioinformatics

Answer 1

Correct choice is (a) True

To elaborate: There has been a great deal of progress in developing computational methods for analyzing genomic sequences and finding these protein-encoding regions. But these methods are not completely reliable and, furthermore, such genomic sequences are often not available.