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Which of the following Fed-batch system is described as a fixed volume?

(a) The same medium used to establish the batch culture

(b) A solution of the limiting substrate

(c) A concentrated solution of the limiting substrate

(d) A very concentrated solution of the limiting substrate

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Right answer is (d) A very concentrated solution of the limiting substrate

To explain I would say: A very concentrated solution of the limiting substrate added at a rate less than the same medium used to establish the batch culture, a solution of the limiting substrate and a concentrated solution of the limiting substrate, resulting in an insignificant increase in volume.

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