Question

Which one of following is not used as a cryoprotectant ?
1. Sucrose
2. DMSO
3. Glycerol
4. Sodium hypochloride

Answer 1

Correct Answer - Option 4 : Sodium hypochloride

The correct answer is Sodium hypochloride.

• Sodium hypochloride is not used as a cryoprotectant.
• A cryoprotectant is a substance used to protect biological tissue from freezing damage.
• Cryoprotectant agents reduce ice formation at any temperature by increasing the total concentration of all the solutes present in the system.
• They play a significant role in cell processing for the storage at deep cryogenic temperatures and cell recovery capacity with a greater level of appropriate functionality.
• Ethylene glycol, dimethyl sulfoxide (DMSO), sucrose, glycerol, etc., are some commonly used cryoprotectants.
• These are sometimes known as “antifreeze” when applied outside the cryobiology.
• A five percent to 15% concentration of cryoprotectants is enough to allow the isolated cells to survive after freezing and thawing from the liquid nitrogen temperature.