Observing mitochondria :
1. Make a fresh solution of Janus Green-B in a beaker.
2. Mix 200 mg Janus Green-B in 100 ml of water.
3. Take a watch glass pour some solution. Put the onion peel in this solution and keep it about half an hour.
4. Keep a piece of onion peel on the slide and wash thoroughly with water. Mitochondria in onion peel ceil
5. Cover the slide with a cover slip and observe it under microscope at high magnification.
Observations :
Green oval or cylindrical grains scattered in the cytoplasm. They are mitochondria.
