Invention of microscope is most important one to find out microorganisms. Basing on this several inventions were made, which break the unsolved problems since 400 years. This credit goes to Antonie van Leeuwenhoek who invented single lens powerful microscope, which could magnify the object 300 times. His curiosity and skill of making powerful lenses were the secrets of this invention of powerful microscope. Later several microbes were identified by using microscopes. This provides a good source to recognize several diseases and treatment by vaccines and antibiotics.