1. Observe a bottle-gourd plant in a garden. It has unisexual flowers i.e. male and female flowers separately.
2. Select 10 female buds of bottle guard, cover with a polythene bag loosely tying the bag on a stalk.
3. Make some tiny holes in the bag with the help of a pin.
4. Two days later observe the buds blooming. (Now collect the pollen grains from a male flower of the Bottle gourd plant).
5. Pluck the stamens of male flower and shake to collect pollen grain in a sheet of paper. Twisting cotton wool over the tip of a match stick prepares a brush.
6. Now uncover five of the ten female flowers. Apply the pollen grain on to the stigma of these flowers with the brush.
7. The pollen grains stick to the stigma.
8. Cover the flowers again with polythene bag. Remove all male flowers from the plant.
9. So that no pollen grain reaches the remaining female flowers.
10. By this experiment we come to know that significant role is played by male flower in the formation of fruit.
11. If transfer of pollen grains take place within the flower it is known as Self Pollination. Transfer of pollen grain from anther of one flower to stigma of another flower of same species is called Cross Pollination.
