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Chymotrypsin catalyses the hydrolysis of proteins containing bulky, aromatic, hydrophobic amino acids. Comment.

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Chymotrypsin catalyses the hydrolysis of proteins containing bulky, aromatic, hydrophobic amino acids. Comment.

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The negatively charged oxygen anion is able to make a nucleophilic attack on the carbonyl carbon of the peptide bond of its substrate. It loosens the carbonyl carbon, so that a water molecule can hydrolyse the bond.

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