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Why is it important to measure biochemical oxygen demand (BOD) of the effluent? At what stage of sewage treatment is this testing done?

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Why is it important to measure biochemical oxygen demand (BOD) of the effluent? At what stage of sewage treatment is this testing done?

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The BOD test measures the rate of uptake of oxygen by microorganisms in a sample of water. It is thus an indicator of pollution level of the water. 
Biological treatment or Secondary treatment

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