Question

ELISA technique is based on the principle of

(A) DNA replication

(B) antigen-antibody interaction

(C) pathogen antigen interaction

(D) antigen protein interaction

Answer 1

(B) antigen-antibody interaction

The principle of ELISA is based on the formation of an antigen-antibody complex. Antibodies in the sample are linked with an enzyme added to the well of the plate, by addition of a specific substrate to the good results in the formation of colored product. In this technique, the sample is incubated in a well of the plate.