Question

The below figure depicts a process used in diagnosis of a disease

A. Identify the mutation and the disease shown in the diagram.

B. Which technique can help in the identification of this technique?

C. Write down the steps occurring during nick translation.

D. How do extent of severity of the disease be detected in the above process?

Answer 1

A. Reciprocal translocation and the disease is CML

B. FISH

C. The enzymes, DNA polymerase I makes DNA and DNase I cuts DNA and are combined in a buffered reaction with dNIP’s including dUTP labelled with a red or green fluorescence.

D. The status of the disease could easily be identified by counting the number of cells, which appeared yellow. Further, it was possible to monitor the effect of chemotherapy and drugs by taking out samples and counting the number of cells appearing yellow.