Question

Describe in brief any two techniques that can be utilised to transfer recombinant DNA into the host cell directly without using any vector.

Answer 1

Techniques that can be utilised to transfer recombinant DNA into the host cell directly without using any vector are as follows:

(a) Micro-injection: rDNA is directly injected into the nucleus of an animal cell.

(b) Biolistics or gene gun: Cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA.