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Mention any two methods of introducing alien DNA into the host.

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  • Recombinant DNA and the bacterial cells are incubated in ice, followed by placing them briefly at 42°C (heat shock) and then putting them back in ice. 
  • By micro injection the recombinant DNA directly injected into the nucleus of the animal cell. 
  • Plant cells are bombarded with high velocity micro – particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun.

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