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Difference between C3 and C4 pathway.

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S.No. Characteristics C3 Pathway C4 Pathway
(i) Cell type One (mesophyll) Two (mesophyll and bundle sheath).
(ii) Kranz anatomy Absent in leaves Present in leaves
(iii) Chloroplasts type One type (granal only) Two types (granal and agranal) Dimorphic
(iv) CO2 acceptor Ribulose 1, 5 bisphosphate (RuBP) Phosphoenolpyruvate (PEP)
(v) First CO2 fixation product 3-PGA (3C compound) Oxaloacetic acid (4C compound).
(vi) Carboxylase enzyme RuBisCO enzyme PEP carboxylase; RuBisCO
(vii) CO2 fixation rate Low High
(viii) O2 inhibition of photosynthesis Present Absent
(ix) Photorespiration High Nil
(x) Productivity Low productivity High productivity
(xi) CO2 compensation point High(25-100μ1 CO2) Low(0-10μ1CO2)
(xii) Temperature optimum 20 to 25º C (Low temperature) 30 to 45°C (High temperature)
(xiii) Examples Rice, wheat and potato etc. Maize, pearl millet, Amaranthus, grass etc.

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