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Answer the following questions: 

(i). List the two methodologies which were involved in human genome project. Mention how they were used.

(ii). Expand ‘YAC’ and mention what was it used for.

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(I) The two methodologies involved in human genome project are: 

i. Expressed Sequence Tags: Identifying all the genes that are expressed as RNA 

ii. Sequence Annotation: Sequencing the whole set of genome coding or non coding sequences and later assigning different region with functions.

(ii) ‘YAC’ stands for Yeast Artificial Chromosome. It is used as a cloning vectors.

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