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List the two methodologies which were involved in human genome project. Mention how they were used.

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Methodologies of Human Genome Project are :

Expressed Sequence Tags (EST's) : This method focuses on identifying all the genes that are expressed as RNA. 

Sequence Annotation : It is an approach of simply sequencing the whole set of genome that contains all the coding and non-coding sequences, and later assigning different regions in the sequence with functions.

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