(a) Micro propagation: This is the propagation of plants involving cell and tissue culture. If vegetative propagation is not possible in a crop, then buds, shoot apex or any other part of the plant can be used as explant for micropropagation.
1. The explants are treated with sterilisation chemicals to prevent microbial growth, and then cultured in a particular nutrient medium.
2. Cells grow and divide to form a cell mass called callus Some growth regulators (Plant hormones) are added.
3. The callus differentiates into plant parts looking like plant (plantlet). After 4-6 weeks the plantlets transferred to the soil.
(b) Bryophyllum: It is a beautiful plant grown in gar xerophyte plant and can grow in any type soil or container. It requires sun and watering time to time. We can grow it vegetatively.
Bryophyllum with growing buds
When a leaf is put on the soil or falls off accidentally from the parent plant. It starts giving out buds in the notches of the leaves. These buds start growing when in touch with soil having moisture. They give out adventitious roots which go into the soil and small aerial shoots which go into the air. So these adventitious buds form many plants from a single plant. Thus we can get many plants from a single leaf. We can grow these tiny plants into separate pots to get independent plants. This is one of the vegetative mode of reproduction.
(c) Vegetative reproduction: This is method of producing new plants from the vegetative parts of the plants. The vegetative part means the leaf, stem, root. As potato, mint, ginger, banana, sugar beet, gul-e-daudi, asparagus, sugar cane, are produced by this method.
(d) Grafting: In plants like mango, zizyphus (ber), guava apples, fruits, roses, a small bud is fixed on the stem. Many types of apples on a single plant thus a small orchard on a single plant. So we can have different types of roses and chrysanthemums on a single plant.
