a. Take a clean grit free slide.
b. Put one drop of normal saline oat one side of the slide & a drop of Lugol’s iodine on another side of the slide.
c. Add on one drop of liquid stool to each of the normal saline & iodine drop. In case of solid or semisolid specimen mix small portion of stool to each of the drop.
d. Cover the preparation with clean, grit free cover slip. Separate cover slips are used for saline & iodine preparation.
e. Observe both the preparation for presence of ova or cyst or live parasites under low & high power objectives of the microscope.
