To evaluate motility, a small drop of liquefied semen is placed on a microscopic slide and then covered with a cover slip and examined under the microscope with low and high power magnification. Motility can be evaluated by scanning several fields with a high dry objective, until a total of at least “200” spermatozoa have been observed. It is essential to focus entire depth of a given field so as to include non-motile sperm that have settled to the bottom of the medium.
The sperm motility is graded as
a) Rapid Progressive motility:
This is the motility along a linear track, covering a distance of at least20 micro mt./ sec ( half the length of the spermatozoa).
b) Slow or sluggish progressive motility:
This is the non linear motility
c) Non- progressive motility: spermatozoa move their tails but do not move forward.
d) Immotility (non- motile).
